LC12A9 Knockout cell line (COS-1)

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LC12A9 Knockout cell line (COS-1) Order

LC12A9 Knockout cell line (COS-1)
Catalog#
YKO-R074
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
LC12A9 Knockout cell line (COS-1)
Order

Detailed Product Information

Product category
KO Cell Lines
cell name
LC12A9 Knockout cell line (COS-1)
Cell Morphology
Epithelial-like, adherent
Passage ratio
1:3-1:4
Species
Green monkey
Gene Name
LC12A9
Gene ID
103246714
Gene Information
NCBI
Construction method
Electroporation/Lentivirus
Mycoplasma test
negative
Culture method
90%DMEM+10%FBS
Target region
Knockout region: Exon 4 (based on transcript SLC12A9-201)
Validation: PCR+Sanger Sequencing
LC12A9 Knockout cell line (COS-1) Target region

Instruction

Use exclusive Red Cotton system to design a sgRNA fragment knockout strategy for the LC12A9 gene, clone the two sgRNAs into CRISPR-U™ vector, transfect the vector into the COS-1 cell line by electroporation/lentivirus method, use the limited dilution method for single-cell cloning and use the EZ-editor™ Monoclone Genotype Validation Kit (Cat# YK-MV-1000) for nucleic acid lysis and PCR amplification on the single-cell clones, followed by Sanger sequencing to verify the genotype. Upon passing the verification, LC12A9 gene knockout LC12A9 Knockout cell line (COS-1) will be expanded and cryopreserved.
Knockout Cell Line Work Flow and Validatio Knockout Cell Line Work Flow and Validatio
LC12A9 Knockout cell line (COS-1)
LC12A9 Knockout cell line (COS-1)
Catalog#
YKO-R074
Size
1*10^6
Storage conditions
Shipped on dry ice; Store in liquid nitrogen
Order

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